Reports had circulated across Spain that the Portuguese star would not feature for los Blancos against their city rivals and assistant coach Aitor Karanka confirmed his absence.
"Cristiano Ronaldo will not be in the squad for tomorrow," he stated at a press conference on Friday.
"With the exception of Cristiano Ronaldo, Marcelo and [Alvaro] Arbeloa, the rest are well and tomorrow our best team will go out to win."
Karanka went on to say that every member of the capital club's squad must give their all against Diego Simeone's side as they look warm up for their Champions League clash with Dortmund on Tuesday.
"Tomorrow we have to be at 120 per cent and go out with the maximum level of intensity," he continued.
"There are still big games left and the team is going to be at that level. We have to go out tomorrow with a winning mentality and increase the points lead [over Atleti].
"The best way to present yourself for Tuesday at the Bernabeu is winning. It's the best way to get motivated."
