He arrived in court in Pretoria wearing a blue suit and a tie. Prosecutors plan to pursue a premeditated murder charge, which he is expected to deny.
Mr Pistorius' girlfriend, model Reeva Steenkamp, was shot dead at his home on Thursday. Police recovered a hand gun.
She is due to be cremated at a private ceremony in Port Elizabeth later.
Mr Pistorius arrived at the court house before his 09:00 (07:00 GMT) hearing, which will be the first since he broke down and wept at an initial hearing on Friday.
Prosecutors are expected to outline their evidence against Mr Pistorius to support their argument that he should be denied bail before his trial begins.
Mr Pistorius is also likely to enter a plea for the first time and apply for bail.
The athlete, who has both legs amputated below the knee, was held at a local police station over the weekend, and has not spoken publicly about Ms Steenkamp's death.
Oscar Pistorius
Popularly known as "blade runner", he was born without a fibula in both legs
Won a key legal battle in 2008, when athletics' governing body, the IAAF, allowed him to compete against able-bodied athletes
Made history in London 2012 by becoming the first amputee sprinter to compete in the Olympics
Apologised after claiming that his rival, Brazilian Alan Oliveira, was wearing blades that were too long in the 2012 Paralympics 200m final
But a statement released by Mr Pistorius's family suggests he will deny the charges being prepared against him.
"The alleged murder is disputed in the strongest terms," it said.
Meanwhile the family of Ms Steenkamp has spoken of its grief in a newspaper interview published on Monday.
Speaking to the Times of South Africa, her mother June Steenkamp said: "All we want are answers... answers as to why this had to happen, why our beautiful daughter had to die like this."
The bail hearing is not expected to be televised after lawyers agreed there should be "no live recording" of proceedings.
The case has aroused huge interest both in South Africa, where he is regarded as a national hero, and around the world.
He rose to fame as one of the most recognisable faces of Paralympic sport, and the first double-amputee track athlete to race in the Olympics.
